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Table 5 Metabolomic analysis of CFCS of Lactobacillus strains Lp03, Lp289 and Lp291

From: Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

Retention time (min) Substance MRS area (%) CFCS area (%) CFCS area (%) CFCS area (%)
Lp03 Lp289 Lp291
4.164 Carbodiimide 1.69 1.70
4.700 N.N-Dimethylglycine 0.04 0.15 0.09
7.295 Lactic acid 0.85 68.16 56.35 67.34
7.524 Acetic acid 0.07 6.52
7.768 Valine 0.05 0.04 1.02
7.936 2-Propenoic acid 0.05 0.11
8.405 Alanine 1.14 1.74 2.38
9.020 Glycine 0.33 0.76 1.11
9.873 β- Lactate 0.08
10.168 Leucine 0.17 0.16 0.07 1.29
10.445 3-Hydroxybutyric acid 0.04 0.26
10.638 α-Hydroxyvaleric acid 0.12 0.16
10.909 Isoleucine 0.79
12.465 Valine 1.02 1.69 2.41 0.15
13.203 4-methyl-2-hydroxypentanoic acid 0.41 0.52 0.58
13.395 3-methyl-2-hydroxypentanoic acid 0.12 0.08 0.06
14.510 Leucine 2.48 2.65 3.79
14.733 Glycerol 0.95
14.769 Phosphoric acid 7.29 7.18 8.32 7.92
15.236 Isoleucine 1.18 1.59 2.44 0.44
15.308 γ- Amino butyric acid 0.03
15.839 Butanoic acid 0.20 0.43 0.57 0.41
16.220 2-methyl-2.3-dihydroxypropanoic acid 0.10 0.16 0.20 0.28
16.705 Pyrimidine 0.05
17.644 Serine 0.75 0.25 0.64 0.61
18.148 Butanoic acid 0.02 0.43 0.09 0.08
18.272 3-Methyl-1.4-dihydroxypiperazine-2.5-dione 0.02
18.496 Butyric acid 0.49 1.53
18.531 Threonine 0.62
18.555 Lactic acid dimer 0.33 0.26 0.77
19.332 2.4-dihydroxybutanoic acid 0.08 0.08
21.091 Trisiloxane 0.13 0.12 0.09
21.769 Malic acid 0.07 0.09
21.865 2-Pyrrolidone-5-carboxylic acid 0.33 0.41 0.41
22.262 Glutamic acid 0.05
22.458 Methionine 0.22
22.588 Proline 1.40 0.89 2.90 0.35
22.708 Aspartic acid 0.56 0.55
23.099 Phenylalanine 0.79 0.44
24.376 Benzenepropanoic acid 0.36 0.53 0.12
25.583 Glutamine 1.76 2.79
26.434 Tartaric acid 0.34 0.35 0.51
26.995 Hydroxy 4-oxo-2.4-di(hydroxyamino)butanoate 0.20
27.048 Asparagine 0.04
27.895 Lysine 0.63
28.638 Arabinitol 0.23
28.805 Ribitol 0.17
29.803 2.3-dihydroxypropylphosphoric acid 0.13 0.24
29.920 D-Ribo-Hexonic acid 0.09
30.946 2-Keto-D-gluconic acid 0.70 0.10
31.133 1.2.3-Propanetricarboxylic acid 4.27 4.33 3.34
31.259 Citric acid 7.49
32.345 Pentanedioic acid 0.23 0.42 0.10
32.451 Benzenepropanoic acid 0.10
32.879 4-Hydroxyphenyllactic acid   0.12
33.891 Tyrosine 0.13 0.05
33.955 Glucitol 0.04 0.18
35.660 Inositol 31.07
39.845 Tryptophan 0.06 0.07
44.979 Uridine 0.09
Identified compounds (except sugars) 60.28 96.94 97.32 94.34
Sugars 38.12 1.14 2.31 5.28
Unidentified compounds 1.60 1.92 0.37 0.38
Total 100.00 100.00 100.00 100.00