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Fig. 3 | BMC Microbiology

Fig. 3

From: Construction and preservation of a stable and highly expressed recombinant Helicobacter pylori vacuolating cytotoxin A with apoptotic activity

Fig. 3

Renaturation of the target recombinant protein by various buffers as detected by SDS-PAGE (left) and western blotting (right). Lane M: Protein marker; Lane 1: Protein before refolding; Lane 2: buffer 1 (50 mM Tris,10% Gly, 150 mM NaCl, pH 8); Lane 3: buffer 2 (50 mM Tris,10% Gly, 150 mM NaCl, 0.1 mM DL-dithiothreitol (DTT), pH 8.0); Lane 4: buffer 3 (1× phosphate buffered saline (PBS), pH 7.4; Lane 5: buffer 4 (1× PBS, 10% GLy, 500 mM NaCl, pH 7.4); Lane 6: buffer 5 (1 × PBS,10% GLy, 500 mM NaCl, 0.1 mM DTT, pH 7.4); Lane 7: buffer 6 (20 mM Tris, 2 M urea, 400 mM Arg, 2.5 mM cysteamine, 0.25 mM cystamine, pH 8.5); Lane 8: buffer 7 (50 mM acetic acid, pH 2.9)

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