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Table 1 FT-IR absorbance spectra and the corresponding functional groups of the monomers observed during polymerization

From: Tyrosinase and laccase-producing Bacillus aryabhattai TFG5 and its role in the polymerization of phenols

Monomers (3 mM)

With standard mushroom tyrosinase

Extracellular protein of B. aryabhattai TFG5

Control (without enzyme)

Catechol

578.5 cm–1: Halogens C-Br Strong-Stretching

584.325 cm–1: Halogen C-Br-strong-stretching

1368.25 cm–1: Alcohol OH Medium-Deformation

1631.5 cm-1: Alkenes C =C Variable-Stretching non conjugated C =C

1637.27 cm–1: Alkenes C =C-Variable-Stretching Non conjugated C =C

1739.48 cm–1: Aldehydes C =O Strong-Stretching saturated aliphatic aldehyde

3327.6 cm–1 : Alcohols OH Variable-Stretching hydrogen-bonded

2156.99 cm–1: Amine salts NH-Medium-Stretching-Secondary amine salt

 
 

3331.43 cm–1: Alcohol OH-Variable-Stretching hydrogen-bonded

 

p-Hydroxy benzoic acid

570.8 cm–1Halogens C-Br Strong-Stretching

429.084 cm–1: Sulfur compounds S-S Variable-Stretching

1368.25 cm–1: Alcohol OH Medium-Deformation

1637.3 cm–1: Alkenes C =C Variable-Stretching non conjugated C =C

1644.02 cm–1: Alkenes NH Variable-Stretching Secondary amine salts

1739.48 cm–1: Aldehydes C =O Strong-Stretching saturated aliphatic aldehyde

3340.1 cm–1 Alcohols OH Variable-Stretching hydrogen-bonded

2162.78 cm–1: Amine salts NH Medium-Stretching Secondary amine salts

 
 

2355.62 cm–1: Amine salts NH Medium-Stretching Tertiary amine salts

 
 

3402.78 cm–1: Amines NH Weak-Secondary amine

 

Ferulic acid

1637.3 cm–1: Alkenes C =C Variable-Stretching non conjugated C =C

556.363 cm–1: Halogens C-Br Strong-Stretching

1637.3 cm–1: Alkenes C =C Variable-Stretching Non conjugated C =C

3340.1 cm–1 : Alcohols OH Variable-Stretching hydrogen-bonded

1325.8 cm–1: Alcohol OH Strong-Deformation

2112.6 cm–1: Alkynes CΞC Variable-Stretching Mono substituted

 

1636.3 cm–1: Alkenes C =C Variable-Stretching Non conjugated C =C

3328.53 cm–1: Alcohol OH Variable-Stretching hydrogen-bonded

 

2156.99 cm–1: Amine salts NH Medium- Stretching Secondary Amine salts

 
 

2338.2 cm–1: Amine salts NH Medium-Stretching Tertiary amine salts

 
 

2369.12 cm–1: Amine salts NH Medium-Stretching Tertiary amine salts

 
 

3331.43 cm–1: Alcohol OH Variable-Stretching Hydrogen Bonded

 

Salicylic acid

1637.3 cm–1: Alkenes C =C Variable-Stretching non conjugated C =C

1214.93 cm–1: Alcohol C-O Strong- Stretching

1637.3 cm–1: Alkenes C =C Variable-Stretching Non conjugated C =C

3340.1 cm–1 : Alcohols OH Variable-Stretching hydrogen-bonded

1367.28 cm–1: Alcohol OH Medium- Deformation

2112.6 cm–1: Alkynes CΞC Variable-Stretching Mono 3328.53substituted

 

1737.55 cm–1: Aldehyde C =O Strong- Stretching saturated aliphatic aldehyde

3328.53 cm–1: Alcohol OH Variable-Stretching hydrogen-bonded

 

2331.52 cm–1: Amine salts NH Medium to strong-stretching tertiary amine salts

 
 

3454.8 cm–1: Amines NH Weak- Stretching Secondary amines

 
 

3627.4 cm–1: Silicon compounds OH Medium-Stretching Broad peak