SaQuant: a real-time PCR assay for quantitative assessment of Staphylococcus aureus

Table 1 In silico assessment of SaQuant and other S. aureus-specific assays

Assay	S. aureus hits (n= 1818)	Other Staphylococci hits (n= 1834)	Sensitivity	Specificity	Reference	Forward	Reverse	Probe	Amplicon size
SaQuant	1738	1	95.60	99.95	This one	AACTACTAGGGGAGCCTAATRAT	GGTACTAACCAAATCAGGTCATAA	TGGCTGAGATGAAYTGTTCAGACCC	73
1	898	1	49.39	99.95	[27]	GGCATATGTATGGCAATTGTTTC	CGTATTGCCCTTTCGAAACATT	ATTACTTATAGGGATGGCTATC	73
2	0	0	0	100	[23]	CAAAGCATCAAAAAGGTGTAGAGA	TTCAATTTTCTTTGCATTTTCTACCA	TTTTCGTAAATGCACTTGCTTCAGGACCA	Unknown
3	753	6	41.42	99.67	[28]	CAGCAAACCATGCAGATGCTA	CGCTAATGATAATCCACCAAATACA	TCAAGCATTACCAGAAAC	101
4	1780	11	97.91	99.40	[29]	AACTGTTGGCCACTATGAGT	CCAGCATTACCTGTAATCTCG		306
5	1499	0	82.45	100	[30]	GCGATTGATGGTGATACGGTT	AGCCAAGCCTTGACGAACTAAAGC	GGTGTAGAGAAATATGGTCCTGAAGCAAGTGCA	279
6^a	1318	1	72.50	99.95	[31]	AATCTTTGTCGGTACACGATATTC	CGTAATGAGATTTCAGTAGATAATACAACA		108
7	117	0	6.44	100	[32]	AGTGAGCGACCATACAACAG	CATAATTCCCGTGACCATTT	AAGCACAAGGACCAATCGAGG	185
femB	1010	0	55.56	100	[33]	TTACAGAGTTAACTGTTACC	ATACAAATCCAGCACGCTCT		651

^aModified sequence to exclude the last 3 nucleotides as the published sequence is not predicted to amplify any S. aureus genomes