Skip to main content

Table 1 Tested strains and plasmids used in this experiment

From: SpoVG is an important regulator of sporulation and affects biofilm formation by regulating Spo0A transcription in Bacillus cereus 0–9

Name

Properties and Application

Source

Strains

B. cereus 0–9

Wild type strain in this study

Kept in our laboratory, isolated from wheat root.

E. coli 116 (pir+)

Plasmid propagation

Purchased from BioVector NTCC

E. coli GM2163 (dam-)

Demethylation

Purchased from BioVector NTCC

 HQ1021

The spoVG gene defect mutant, ∆spoVG

Construct in this study

 ZY1001

The abrB gene defect mutant, ∆abrB

Construct in this study

 FPU1061I

The sinI gene defect mutant, ∆sinI

Construct in this study

 FPU1062R

The sinR gene defect mutant, ∆sinR

Construct in this study

 ZL1002

The spo0A gene defect mutant, ∆spo0A

Construct in this study

 HQ2021

The double knockout strain of spoVG and sinI genes, ∆spoVGsinI

Construct in this study

 HQ2022

The double knockout strain of spoVG and sinR genes, ∆spoVGsinR

Construct in this study

 HQ2023

The double knockout strain of spoVG and abrB genes, ∆spoVGabrB

Construct in this study

 HQ2024

The double knockout strain of spoVG and spo0A genes, ∆spoVGspo0A

Construct in this study

 HQ7110

ΔspoVG supplemented with its native spoVG gene by allelic exchange, ∆spoVG::spoVG

Construct in this study

 HQ7170

ΔspoVG supplemented with spoVG gene from B. subtilis 168 by allelic exchange, ∆spoVG::spoVG 168

Construct in this study

 HQ7121

HQ1021 supplemented with its native spo0A gene by reverse complementary, ∆spoVG/spo0A

Construct in this study

 HQ7221

HQ2024 supplemented with its native spo0A gene by reverse complementary, ∆spoVGspo0A/spo0A

Construct in this study

 HQ7222

HQ2024 supplemented with its native spoVG gene by reverse complementary, ∆spoVGspo0A/spoVG

Construct in this study

 HQg6071

B. cereus 0–9 with PexsY-GUS labeling

Construct in this study

 HQg6171

HQ1021 (ΔspoVG) with PexsY-GUS labeling

Construct in this study

 HQg6072

Wild B. cereus 0–9 with PsipW-GFP labeling

Construct in this study

 HQg6172

HQ1021 with PsipW-GFP labeling

Construct in this study

 HQg6073

Wild B. cereus 0–9 with PcalY-GFP labeling

Construct in this study

 HQg6173

HQ1021with PcalY-GFP labeling

Construct in this study

 HQg6074

Wild B. cereus 0–9 with PabrB-GFP labeling

Construct in this study

 HQg6174

HQ1021 with PabrB-GFP labeling

Construct in this study

 HQg6175

Wild B. cereus 0–9 with PsinI-GFP labeling

Construct in this study

 HQg6175

HQ1021 with PsinI-GFP labeling

Construct in this study

Plasmid

 pAD

For gene knockout

Takara, Dalian

spoVG-pMAD

For gene knockout, Amp+; Erm+

Construct in this study

abrB-pMAD

For gene knockout, Amp+; Erm+

Construct in this study

 pAD-pgal-JT

For reverse complementation

Stored in our laboratory

 pMAD-chi

For gene complementation, Cm+

Construct in this study

 pET28a-gapB

Expression of protein, Km+

Construct in this study