Table 1 Kinetic and thermodynamic parameters of fungal Tpi1 binding by ECM proteins, determined by SPR measurements
ECM protein | ka (1/Ms) | kd (1/s) | KD (M) |
|---|---|---|---|
C. albicans Tpi1 | |||
VTR | 1.02 x 105 ± 3.16 x 103 | 8.77 x 10-3 ± 7.40 x 10-4 | 8.60 x 10-8 ± 5.28 x 10-9 |
FN | 3.22 x 104 ± 3.99 x 103 | 1.05 x 10-2 ± 5.08 x 10-4 | 3.26 x 10-7 ± 2.73 x 10-8 |
LAM | 4.42 x 104 ± 4.09 x 103 | 3.27 x 10-2 ± 1.68 x 10-3 | 7.39 x 10-7 ± 7.99 x 10-8 |
COL | 1.82 x 104 ± 2.48 x 103 | 1.99 x 10-3 ± 3.10 x 10-4 | 1.09 x 10-7 ± 9.16 x 10-9 |
ELA | 4.65 x 104 ± 7.79 x 103 | 3.82 x 10-2 ± 1.56 x 10-3 | 8.21 x 10-7 ± 8.04 x 10-8 |
C. glabrata Tpi1 | |||
VTR | 1.84 x 104 ± 9.84 x 102 | 3.10 x 10-3 ± 1.04 x 10-3 | 1.68 x 10-7 ± 4.13 x 10-9 |
FN | 6.48 x 104 ± 5.23 x 103 | 4.73 x 10-2 ± 1.36 x 10-3 | 7.30 x 10-7 ± 7.24 x 10-9 |
LAM | 1.34 x 104 ± 4.05 x 103 | 3.39 x 10-3 ± 2.62 x 10-4 | 2.53 x 10-7 ± 3.18 x 10-8 |
COL | 1.44 x 104 ± 1.90 x 103 | 3.08 x 10-3 ± 2.91 x 10-4 | 2.14 x 10-7 ± 2.86 x 10-8 |
ELA | 9.51 x 104 ± 4,83 x 103 | 1.91 x 10-3 ± 9.04 x 10-5 | 2.01 x 10-8 ± 1.97 x 10-8 |