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Table 1 Kinetic and thermodynamic parameters of fungal Tpi1 binding by ECM proteins, determined by SPR measurements

From: Candida albicans and Candida glabrata triosephosphate isomerase – a moonlighting protein that can be exposed on the candidal cell surface and bind to human extracellular matrix proteins

ECM protein ka (1/Ms) kd (1/s) KD (M)
C. albicans Tpi1
 VTR 1.02 x 105 ± 3.16 x 103 8.77 x 10-3 ± 7.40 x 10-4 8.60 x 10-8 ± 5.28 x 10-9
 FN 3.22 x 104 ± 3.99 x 103 1.05 x 10-2 ± 5.08 x 10-4 3.26 x 10-7 ± 2.73 x 10-8
 LAM 4.42 x 104 ± 4.09 x 103 3.27 x 10-2 ± 1.68 x 10-3 7.39 x 10-7 ± 7.99 x 10-8
 COL 1.82 x 104 ± 2.48 x 103 1.99 x 10-3 ± 3.10 x 10-4 1.09 x 10-7 ± 9.16 x 10-9
 ELA 4.65 x 104 ± 7.79 x 103 3.82 x 10-2 ± 1.56 x 10-3 8.21 x 10-7 ± 8.04 x 10-8
C. glabrata Tpi1
 VTR 1.84 x 104 ± 9.84 x 102 3.10 x 10-3 ± 1.04 x 10-3 1.68 x 10-7 ± 4.13 x 10-9
 FN 6.48 x 104 ± 5.23 x 103 4.73 x 10-2 ± 1.36 x 10-3 7.30 x 10-7 ± 7.24 x 10-9
 LAM 1.34 x 104 ± 4.05 x 103 3.39 x 10-3 ± 2.62 x 10-4 2.53 x 10-7 ± 3.18 x 10-8
 COL 1.44 x 104 ± 1.90 x 103 3.08 x 10-3 ± 2.91 x 10-4 2.14 x 10-7 ± 2.86 x 10-8
 ELA 9.51 x 104 ± 4,83 x 103 1.91 x 10-3 ± 9.04 x 10-5 2.01 x 10-8 ± 1.97 x 10-8
  1. The binding parameters for interactions between fungal proteins and human ECM proteins were determined after global fitting of the data with a 1:1 Langmuir binding model. All parameters are presented with standard errors