Fig. 6From: Candida albicans and Candida glabrata triosephosphate isomerase – a moonlighting protein that can be exposed on the candidal cell surface and bind to human extracellular matrix proteinsSPR sensograms for the interactions between candidal Tpi1 and human VTR. VTR solutions in a buffer containing 10 mM HEPES, 150 mM NaCl and 0.005 % (w/v) P20 surfactant, pH 7.4, at concentrations in a range of 50-750 nM, were injected onto a CM5 chip containing immobilized Tpi1 (150 RU) at a flow rate of 30 μL/min for 180 seconds. A 1:1 Langmuir binding model was fitted to the experimental data (black lines)Back to article page