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Fig. 2 | BMC Microbiology

Fig. 2

From: Candida albicans and Candida glabrata triosephosphate isomerase – a moonlighting protein that can be exposed on the candidal cell surface and bind to human extracellular matrix proteins

Fig. 2

Identification of Tpi1 on the surface of C. albicans and C. glabrata cells grown under various conditions. For C. albicans cells, the experiment was performed in the wells of MaxiSorp microplates (1 x 106 cells per well) whereas for C. glabrata cells, the experiment was carried out in Eppendorf tubes (3 x 107cells per tube). The cells were grown under different culture conditions., i.e. YPD, YPDA or RPMI 1640, and were incubated with an anti-yeast Tpi1 antibody (1 µg/mL) followed by a HRP-conjugated secondary antibody. After washing off any unbound material, the level of bound antibodies was determined using TMB; however, in the case of C. albicans cultured in YPD medium the fluorescence signals were not measurable (as shown in Figure S2 in the Supplementary material). The obtained results were normalized for the same number of cells (1 x 106) and for the different cross-reactivities of the applied anti-S. cerevisiae Tpi1 antibody with C. glabrata and C. albicans (based on the calibration presented in Figure S1A in the Supplementary material). Bars represent the mean values from three determinations, with standard deviations. Statistical significance levels were determined by one-way ANOVA; **p<0.01 and ****p<0.0001

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