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Fig. 1 | BMC Microbiology

Fig. 1

From: Candida albicans and Candida glabrata triosephosphate isomerase – a moonlighting protein that can be exposed on the candidal cell surface and bind to human extracellular matrix proteins

Fig. 1

Identification of Tpi1 on the surface of C. albicans and C. glabrata cells grown in RPMI 1640 medium. C. albicans yeast-like cells, grown for 16 h in YPD medium at 30°C, were transferred to the wells of a glass bottom 96-well plate coated with poly-L-lysine (104 cells per well) and incubated in RPMI 1640 medium at 37 °C overnight. C. glabrata yeast-like cells were grown overnight in RPMI 1640 medium in a glass flask and then transferred to Eppendorf tubes (3 x 107 cells per tube). The presence of surface-exposed Tpi1 was detected using rabbit polyclonal anti-Tpi antibody (1 µg/mL) and an Alexa Fluor 488 fluorescently labeled anti-rabbit secondary antibody (1 μg/mL). The photos were taken using an Olympus IX73 microscope

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BMC Microbiology

ISSN: 1471-2180

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