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Fig. 2 | BMC Microbiology

Fig. 2

From: Optimisation of sample storage and DNA extraction for human gut microbiota studies​

Fig. 2

Comparison of different starting material sources (M—suspension, P—pellet, S—supernatant) and two commercial stool DNA extraction kits (PL—PureLink™ Microbiome DNA Purification Kit, ZR—ZymoBIOMICS™ DNA Miniprep Kit) for extracted gDNA (A) concentration and (B) purity at OD 260/230. Asterisks indicate statistical significance determined by Student’s t-test: *** (p < 0.001), ** (p < 0.01), * (p < 0.05), and n/s not significant. (C) Non-metric multidimensional scaling of samples (S1–S5 represent individuals) in response to starting material and isolation kit used. Ellipses indicate distinct clusters of every individual with 95 % confidence

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