Table 6 qPCR primers used in this study
Target | Primers | Sequence (5' to 3') | Tm (ËšC) | % GC | amplicon size (bp) |
|---|---|---|---|---|---|
Forward and reverse primers targeting whiB7 (BB28_RS17590) | WhiB7_qPCR_F4 | ACTTTCCGCGAACCACAG | 55.6 | 55.6 | 81 |
WhiB7_qPCR_R1a | ATGATGACCGTCGAAGTGG | 54.6 | 52.6 | ||
Forward and reverse primers targeting 16s rRNA (BB28_RS07070) | Myco_16S_F1 | CCGGATAGGACCACACACTT | 56.6 | 55 | 91 |
Myco_16S_R1 | ATTACCCCACCAACAAGCTG | 55.4 | 50 |