Fig. 6
The average relative expression levels of whiB7 in M. chelonae carrying McProf alone, no prophage, BPs alone, or BPs and McProf as measured with SYBR Green quantitative RT-PCR. a. RNA was harvested in triplicate from strains grown to an OD600 of 0.7 before treating or not treating with 75 μM ACI and incubating for an additional 3 h. The culture OD600 at time of harvest was close to 1 but differed slightly for each strain and treatment. b. RNA was harvested in triplicate from strains grown to an OD600 of 0.9 before treating or not treating with 16.7 μM AMK and incubated for an additional 1 h. Graphs represent average values ± standard error of the mean with n = 3. Means with different letters are significantly different (Tukey’s HSD, α = 0.05)