Table 3 Oligonucleotides sequences used in this study
Target | Primer sequence (5′-3′) | Reference |
|---|---|---|
q-RT-PCR | ||
 Bacteroidetes | Forward – CRAACAGGATTAGATACCCT Reverse – GGTAAGGTTCCTCGCGTAT | [81] |
 Firmicutes | Forward – TGAAACTYAAAGGAATTGACG Reverse – ACCATGCACCACCTGTC | [82] |
 ỿ-Proteobacteria | Forward - TCGTCAGCTCGTGTYGTGA Reverse – CGTAAGGGCCATGATG | [82] |
 Eubacteria | Forward – ACTCCTACGGGAGGCAGCAGT Reverse - ATTACCGCGGCTGCTGGC | [83] |
 Bacteroides spp. | Forward – CGATGGATAGGGGTTCTGAGAGGA Reverse - GCTGGCACGGAGTTAGCCGA | [50] |
 Prevotella spp. | Forward – CACCAAGGCGACGATCA Reverse - GGATAACGCCYGGACCT | [50] |
 Bifidobacterium spp. | Forward- TCGCGTC(C/T)GGTGTGAAAG Reverse - CCACATCCAGC(A/G)TCCAC’ | [84] |
 Lactobacillus spp. | Forward - AGCAGTAGGGAATCTTCCA Reverse – CACCGCTACACATGGAG | [84] |
 stx2a | Forward – ACCCCACCGGGCAGTT Reverse - GGTCAAAACGCGCCTGATA | [39] |
 rpoA | Forward – GCGCTCATCTTCTTCCGAAT Reverse - CGCGGTCGTGGTTATGTG | [55] |
PCR | ||
 stx2a | Forward – CAGTCGTCACTCACTGGTTTCATCA Reverse - GGATATTCTCCCCACTCTGACACC | [85] |