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Table 2 Feed and pancreatic juice composition used in the SHIME®

From: Human microbiota modulation via QseC sensor kinase mediated in the Escherichia coli O104:H4 outbreak strain infection in microbiome model

Feed Componenta

g/L

Arabinogalactan

1.0

Pectin

2.0

Xylan

1.0

Potato starch

3.0

Glucose

0.4

Yeast extract

3.0

Peptone

1.0

Mucin

4.0

Cystein

0.5

Sterile distilled water

q.s.pb

Pancreatic juice

Oxgall

6

NaHCO3

12.5

Pancreatin

0.9

Sterile distilled water

q.s.p

  1. a Sigma-Aldrich
  2. b Quantum Satis para (for Liter)