Table 1 Sequences of primers in this study
Primers | sequence 5’-3’ (Forward/Reverse) | Reference |
|---|---|---|
RT-qPCR | ||
icaA | 5’- GATTATGTAATGTGCTTGGA -3’/ | Ref. [30] |
5’- ACTACTGCTGCGTTAATAAT - 3’ | ||
yycF | 5’ - TGGCGAAAGAAGACATCA -3’/ | Ref. [30] |
5’ – AACCCGTTACAAATCCTG- 3’ | ||
yycG | 5’ - CGGGGCGTTCAAAAGACTTT -3’/ | Ref. [30] |
5’ - TCTGAACCTTTGAACACACGT -3’ | ||
sarA | 5’ - AGATGGCCCTTCTTCAAATG -3’/ | This study |
5’ –CCGCAATAATTCTTGTGACG -3’ | ||
codY | 5’ - GGTGGAGGGGAAAGATTAGG -3’/ | This study |
5’ –GCGCGCTTCTTTTTCTACTT -3’ | ||
16S rRNA | 5’ - GTAGGTGGCAAGCGTTATCC -3’/ | Ref. [30] |
5’ –CGCACATCAGCGTCAACA-3’ | ||
EMSA assay | ||
promoter regions of sarA | 5’ - GCGCAATTTGGTGAAGTTTGATAGATG -3’/ | This study |
5’ –GTGATATATAAACCTAGGGCATAAAGTCC -3’ | ||
promoter regions of icaA | 5’ - CTGAAAATTAATCACACTATGTTACAGG -3’/ | This study |
5’ – CTTTACCTACCTTTCGTTAGTTAGGTTG -3’ | ||