Fig. 5

EMSA for sarA and icaA promoter regions. a Consensus YycF binding motif and candidate sequences in promoters of sarA. TGTWAH-NNNNN-TGTWAH, where W is A/T and H is A/T/C; b EMSA in which promoter regions were obtained by PCR and FAM-labelled. Excess cold (unlabeled) promoter DNAs were used to out-compete YycF- specific binding. As the negative control, a DNA fragment the same size as the promoter region and similar AT: GC mole ratio, but missing the YycF consensus binding sequence, was used to rule out non-specific binding; c The working model of oxidation regulation by ASyycG via sarA pathway