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Fig. 4 | BMC Microbiology

Fig. 4

From: Antisense yycG modulates the susceptibility of Staphylococcus aureus to hydrogen peroxide via the sarA

Fig. 4

The AS yycG overexpression inhibited the transcripts of biofilm-related genes. a Biomass was quantified by crystal violet staining. Optical densities at 600 nm were measured (n = 10, *P < 0.05); b Quantitative RT-PCR analysis showed the gene transcripts in S. aureus, and ASyycG strains treated with H2O2. S. aureus gene expression was relatively quantified by RT-PCR using 16 S as an internal control (n = 10, *P < 0.05); c The productions of YycF and YycG were quantified in the cells of S. aureus, and ASyycG strains treated with H2O2 for Western blotting (upper lane). The lower panel shows a Coomassie-stained gel supporting equal loading of the samples for total bacterial lysis; d The purified recombinant YycF protein was visualized by Coomassie staining after SDS-PAGE

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