Fig. 4

B. pseudomallei induces ER stress in RAW264.7 cells. a Representative TEM images of RAW264.7 cells after uninfected or infected with B. pseudomallei (MOI = 10) for 8 h. White arrowheads indicate the B. pseudomallei, and black arrowheads indicate the ER. b RAW264.7 cells were uninfected or infected with B. pseudomallei (MOI = 10) for 8 h. Cells were stained with anti-calreticulin antibody (red), anti-B. pseudomallei antibody (green) or DAPI (blue). Scale bar is 10 μm. c The mRNA expression of Bip, CHOP, in RAW264.7 cells infected with B. pseudomallei (MOI = 10) for 4, 8 and 12 h. The ANOVA with a post-hoc test was used to analyze multiple time points. d Immunoblot analysis of Bip, CHOP, elF2α and p-elF2α levels after B. pseudomallei infection (MOI = 10) for 4, 8 and 12 h. e Cells were infected with 10 MOI for 4, 8 and 12 h, and processed for RNA. XBP1 spliced and unspliced mRNA species were resolved by 2.5% agarose gel. The full-length blots or gels are presented in Supplementary Fig. S4 and S5. Results were measured at least 3 times. *P < 0.05, **P < 0.01, ***P < 0.001