Fig. 3

B. pseudomallei infection induces apoptosis of RAW264.7 cells. a RAW264.7 cells were infected with B. pseudomallei (MOI = 10) for 4, 8 and 12 h, and then stained with Annexin V-FITC/PI and analyzed by flow cytometry. b The percentage of early apoptotic cells relative to the total number of cells was counted. c Measurement of the caspase-3 and cleaved caspase-3 in RAW264.7 cells subjected to the indicated treatments by western blot analysis. The full-length blots are presented in Supplementary Fig. S4. The data obtained are at least 3 independent experiments. The ANOVA with a post-hoc test was used to analyze multiple time points. **P < 0.01, ***P < 0.001