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Table 2 Bacterial strains and plasmids used in this study

From: Investigation of the invasion mechanism mediated by the outer membrane protein PagN of Salmonella Typhimurium

Strain or Plasmid Relevant characteristeristics Source or reference
Strains
 HB101 Noninvasive laboratory strain (supE44 hsdS20(rBmB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1) Promega
 BL21 pLysS An E. coli strain which is lysogenic for λ-DE3 and contains the T7 bacteriophage gene I, encoding T7 RNA polymerase under the control of the lac UV5 promoter as well as a plasmid, pLysS, which carries the gene encoding T7 lysozyme (Cm r) Promega
 MC1061 E. coli hsdR mcrB araD139 Δ (araABC-leu)7679 ΔlacX74 galU galK rpsL thi [76]
Plasmids
 pSUP202 pMB1 replicon (Cbr, Tcr, Cmr) [77]
 pSUP202-Rck Vector carrying the rck gene (Cbr, Cmr) [14]
 pSUP202-PagN Vector carrying the pagN gene (Cbr, Cmr) This study
 pSUP202-Inv GFP Vector carrying the invasin gene from Yersinia enterocolitica and gfp gene (Cbr) [14]
 pGEX-4 T-2 Fusion vector carrying the glutathione S-transferase gene (Cbr) GE Healthcare
 pGEX-4 T-2114–159 Rck Vector carrying the glutathione S-transferase (GST) gene linked to 113–159 rck gene (Cbr) [10]
 pGEX-4 T-2 PagN Vector carrying the glutathione S-transferase (GST) gene linked to pagN gene (Cbr) This study
 pcDNA 3.1 Wp85 Vector carrying the wild-type bovine p85α sequence (Cbr) [12]
 pcDNA 3.1 ∆p85 Vector carrying the mutant bovine p85α sequence (Cbr) [12]
  1. Cbr carbenicillin resistance, Tcr tetracyclin resistance, Cmr chloramphenicol resistance