Table 2 Bacterial strains and plasmids used in this study

 HB101

Noninvasive laboratory strain (supE44 hsdS20(r_B⁻m_B⁻) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1)

Promega

 BL21 pLysS

An E. coli strain which is lysogenic for λ-DE3 and contains the T7 bacteriophage gene I, encoding T7 RNA polymerase under the control of the lac UV5 promoter as well as a plasmid, pLysS, which carries the gene encoding T7 lysozyme (Cm ^r)

Promega

 MC1061

E. coli hsdR mcrB araD139 Δ (araABC-leu)7679 ΔlacX74 galU galK rpsL thi

[76]

 pSUP202

pMB1 replicon (Cb^r, Tc^r, Cm^r)

[77]

 pSUP202-Rck

Vector carrying the rck gene (Cb^r, Cm^r)

[14]

 pSUP202-PagN

Vector carrying the pagN gene (Cb^r, Cm^r)

This study

 pSUP202-Inv GFP

Vector carrying the invasin gene from Yersinia enterocolitica and gfp gene (Cb^r)

[14]

 pGEX-4 T-2

Fusion vector carrying the glutathione S-transferase gene (Cb^r)

GE Healthcare

 pGEX-4 T-2114–159 Rck

Vector carrying the glutathione S-transferase (GST) gene linked to 113–159 rck gene (Cb^r)

[10]

 pGEX-4 T-2 PagN

Vector carrying the glutathione S-transferase (GST) gene linked to pagN gene (Cb^r)

This study

 pcDNA 3.1 Wp85

Vector carrying the wild-type bovine p85α sequence (Cb^r)

[12]

 pcDNA 3.1 ∆p85

Vector carrying the mutant bovine p85α sequence (Cb^r)

[12]