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Fig. 9 | BMC Microbiology

Fig. 9

From: Investigation of the invasion mechanism mediated by the outer membrane protein PagN of Salmonella Typhimurium

Fig. 9

Protein tyrosine kinases are required for Rck- and PagN-mediated internalization. CHO (a-b) or Jeg-3 (c-d) cells were incubated with genistein at the indicated concentrations for 15 min prior to the addition of HB101-pagN (a-b) or MC1061-rck (c-d) at MOI 1:10 for 1 h at 37 °C. Percentages of total cell-associated bacteria (a-c: grey bars) and internalized bacteria (b-d: white bars) were determined as described in the Materials and Methods. Results obtained with drugs are expressed relative to values obtained for the same amount of DMSO-containing medium (DMSO), arbitrarily set at 100%. Data were compared using a Mann Whitney test **p < 0.01, *p < 0.05)

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