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Fig. 3 | BMC Microbiology

Fig. 3

From: Investigation of the invasion mechanism mediated by the outer membrane protein PagN of Salmonella Typhimurium

Fig. 3

The presence of heparan sulfate is not correlated to the level of PagN-mediated internalization. a The distribution of HS was analyzed on the cell surface of CHO, pgsA745, Caco-2 and HT29 cell lines by flow cytometry using a specific anti-HS antibody. The percentage of HS positive (HS+) cells was calculated using histogram subtraction (method of Overton) for each cell line as described in the Materials and Methods. b CHO, pgsA745 and Caco-2 cells were infected with HB101-pagN strain at 37 °C for 1 h (MOI 1:10). The percentage of internalized bacteria was calculated as described in Materials and Methods and related to values obtained for CHO cells, set at 100%. Results represent mean values ± SD obtained from three independent experiments. Results were compared using a Mann Whitney test (***p < 0.001, **p < 0.01)

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