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Fig. 2 | BMC Microbiology

Fig. 2

From: Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast

Fig. 2

Relative translation activity of K. phaffii CBS2612 at different specific growth rates measured by OPP-labelling. All cultivations were done in minimal media, either in glucose-excess (ASMv6 with 2% glucose) or in glucose-limited conditions (ASMv6 containing a polysaccharide and a glucose-releasing enzyme). In the glucose-limited conditions a controlled decrease of growth rate over time is taking place, therefore incubation time determined the obtained growth rates. Cells were treated with Imipramine and 0.3 g L− 1 OPP, click labelled with AF488 and the fluorescence signal measured with flow cytometry. a OPP-assay of cells treated with 0.15 g L− 1 Imipramine. Cells at μ = 0 were cultivated in excess glucose with addition of 20 g L− 1 hygromycin to inhibit translation activity. The shown fluorescence signals were subtracted by a no-OPP control and are relative to the signal measured in glucose-excess conditions, which was set to 100. b OPP assay of cells treated with different Imipramine concentrations. c OPP assay of cells treated with 1.5 g L− 1 or no Imipramine. The values shown in b) and c) consist of the received fluorescence signal relative to the respective no-OPP control and therefore are presented as fold change

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