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Fig. 3 | BMC Microbiology

Fig. 3

From: Reproducible and accessible analysis of transposon insertion sequencing in Galaxy for qualitative essentiality analyses

Fig. 3

TraDIS - Comparison of methods for genes classification by regression analysis. a UpSet plot comparing the essential genes predicted by each threshold with the curated list. There is little difference between the two thresholds for essential gene prediction. 341 essential genes are identified in the three sets. Using a threshold of 4 predicts five additional genes compared to a threshold of 12, four of which are also identified in the curated list. b UpSet plot comparing the non-essential genes predicted by each threshold with the curated list. When looking at the non-essential gene list, we see that using a threshold of 12 misses 1570 non-essential genes identified in the paper and a regression using a threshold of 4. The latest predicts 140 non-essential genes that are unclassified in the two other sets. c UpSet plot comparing the non-essential genes predicted by using hand-fit curves, automated fit with Bio-TraDis, and the curated list. The two regression methods identify 344 of the essential genes predicted by the publication. One gene is missed by the two methods, and eight are identified by only one of the regression analyses

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