Fig. 2

Scattered colony pattern of Pseudanabaena sp. NIES-4403 on solid BG-11 media. a. Time-lapse images of Pseudanabaena on a 90-mm plate. Hour 0 represents time that time-lapse photography started, corresponding to ~ 10 h after inoculating a cell suspension at the center of the plate. Time-lapse imaging was performed every hour, and the images were compiled into Movies S1. b. Trajectory of wandering colonies over 120 h from hour 0 of Movie S1. Color represents the passing count (log) (1 count, cyan; 120 count, magenta). c. A kymograph of colonies represented by yellow lines shown in panel a over a 200-h period (top, hour 0; bottom, hour 200). Cyan and magenta triangles represent a rotating disk and a comet-like wandering cluster, respectively. d. Colony size distribution inside the red dotted circle in panel a at hours 100 and 200 on a log-log plot. e. Cumulative distribution function of colony size at hours 100 and 200. It should be noted that the largest cluster located at the center is not considered because it is derived from the initial spot of inoculation; thus, its morphological pattern is due to both autonomous behavior and the artificial inoculation setting