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Table 8 Primer sequences for amplification of MBL and AmpC genes

From: Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria

Gene targets Primer sequence (5′ to 3′, as synthesized) Expected amplicon size (bp) Reference
MBL genes
blaIMP-1 F1 (5′-ACCGCAGCAGAGTCTTTGCC-3′) R1 (5′-ACAACCAGTTTTGCCTTACC-3′) 587 [39]
blaIMP-2 F2 (5′-GTTTTATGTGTATGCTTCC-3′) R2 (5′-AGCCTGTTCCCATGTAC-3′) 678 [39]
blaVIM-1 F3 (5′-AGTGGTGAGTATCCGACAG-3′) R3 (5′-ATGAAAGTGCGTGGAGAC-3′) 261 [39]
blaVIM-2 F4 (5′-ATGTTCAAACTTTTGAGTAAG-3′) R4 (5′-CTACTCAACGACTGAGCG-3′) 801 [39]
AmpC genes
blaCMY F1 (5′-GCTGCTCAAGGAGCACAGGAT-3′) 520 [40]
R1 (5′-CACATTGACATAGGTGTGGTG-3′)
blaDHA F1 (5′-AACTTTCACAGGTGTGCTGGGT-3′) 405 [40]
R1 (5′-CCGTACGCATACTGGCTTTGC-3′)
blaACC F1 (5′-AACAGCCTCAGCAGCCGGTTA-3′) 346 [40]
R1 (5′-TTCGCC GCAATCATCCCTAGC-3′)
blaFOX F1 (5′-AACATG GGGTATCAGGGAGATG-3′) 190 [40]
R1 (5′-CAAAGCGCGTAACCGGATTGG-3′)