Metallo-β-lactamase and AmpC genes in Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolates from abattoir and poultry origin in Nigeria

Table 8 Primer sequences for amplification of MBL and AmpC genes

Gene targets	Primer sequence (5′ to 3′, as synthesized)	Expected amplicon size (bp)	Reference
MBL genes
bla_IMP-1	F1 (5′-ACCGCAGCAGAGTCTTTGCC-3′) R1 (5′-ACAACCAGTTTTGCCTTACC-3′)	587	[39]
bla_IMP-2	F2 (5′-GTTTTATGTGTATGCTTCC-3′) R2 (5′-AGCCTGTTCCCATGTAC-3′)	678	[39]
bla_VIM-1	F3 (5′-AGTGGTGAGTATCCGACAG-3′) R3 (5′-ATGAAAGTGCGTGGAGAC-3′)	261	[39]
bla_VIM-2	F4 (5′-ATGTTCAAACTTTTGAGTAAG-3′) R4 (5′-CTACTCAACGACTGAGCG-3′)	801	[39]
AmpC genes
bla_CMY	F1 (5′-GCTGCTCAAGGAGCACAGGAT-3′)	520	[40]
bla_CMY	R1 (5′-CACATTGACATAGGTGTGGTG-3′)	520	[40]
bla_DHA	F1 (5′-AACTTTCACAGGTGTGCTGGGT-3′)	405	[40]
bla_DHA	R1 (5′-CCGTACGCATACTGGCTTTGC-3′)	405	[40]
bla_ACC	F1 (5′-AACAGCCTCAGCAGCCGGTTA-3′)	346	[40]
bla_ACC	R1 (5′-TTCGCC GCAATCATCCCTAGC-3′)	346	[40]
bla_FOX	F1 (5′-AACATG GGGTATCAGGGAGATG-3′)	190	[40]
bla_FOX	R1 (5′-CAAAGCGCGTAACCGGATTGG-3′)	190	[40]

ISSN: 1471-2180