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Fig. 4 | BMC Microbiology

Fig. 4

From: Proteomic response of Escherichia coli to a membrane lytic and iron chelating truncated Amaranthus tricolor defensin

Fig. 4

Atr-DEF2(G39-C54) validation experiments. (a) E. coli NPN uptake after treatment with Atr-DEF2(G39-C54). (b) Ferric reducing potential of Atr-DEF2(G39-C54) measured via FRAP assay. (c) Fe2+ chelating activity of Atr-DEF2(G39-C54) measured via decreased formation of UV-absorbing Fe2+-ferrozine complex and normalized to the chelating activity of EDTA. P value: *, p < 0.05 compared to negative control; **, p < 0.01; ***, p < 0.0001; n = 3

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BMC Microbiology

ISSN: 1471-2180

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