Fig. 4

Predicted function of microbiome at the various stages of liver disease. Predicted microbial function assessed by KEGG annotation demonstrating a shift in microbial function with progression of liver injury/disease (baseline/WT, n = 6; liver inflammation/Mdr2−/−, n = 6; liver cirrhosis/Mdr2−/−, n = 9 and hepatocellular carcinoma (HCC)/Mdr2−/−, n = 10). Data presented as relative gene count (%); box plots indicate median (middle line), 25th, 75th percentile (box) and 10th and 90th percentile (whiskers) as well as outliers (single points). Differences in relative abundance were assessed by Kruskal-Wallis for overall comparison and Dunn’s test for 2 group comparison with Benjamini-Hochberg multiple test correction. *P < 0.05; ** P < 0.01; *** P < 0.001 and **** P < 0.0001