Fig. 1
From: The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal
Bifidobacterium dentium is resistant to acid stress. a Representative images of live/dead staining of B. dentium ATCC 27678 after 2 h incubation in media at pH 7, 6, 5, 4, and 3. Inserts at high magnification highlight the abundance of live (green) and dead (red) B. dentium at pH 7 and pH 3 (scale bar = 50 μm). b Quantitation of live/dead cell staining on a fluorescent plate reader. c Intracellular pH analysis of B. dentium with pHrodo Red pH sensitive dye. Variance in intracellular pH is reflected by the change in relative fluorescence units (RFU), at various extracellular pH values over 50 min. d Calculated final intracellular pH values at t = 50 min. All data are presented as mean ± stdev