Fig. 9

Cytokine secretion assay. J774A.1 cells were cultured in the presence of different amounts of GroEL protein and 50 ng/mL LPS treated and untreated with 50 μg/mL of polymyxin B. The concentrations of interleukin-10 (IL-10), interleukin-17A (IL-17A), tumor necrosis factor (TNF-α), interferon-γ (IFN-γ), interleukin-6 (IL-6), interleukin-4 (IL-4) and interleukin-2 (IL-2), in culture media, were measured by flow cytometry. Cells were stimulated with 0 (medium), 1, 5 and 10 μg/mL of GroEL after 20 h of incubation at 37 °C. Data represent the mean of cytokine concentrations in the cellular supernatant ± standard error of three independent experiments. Values of p < 0.05 (*) were considered statistically significant, when compared to the binding of the cells did not stimulate (medium) by t-test