Fig. 7

Competitive binding essays. Different amounts of LigBC (0–1 μM) and a fixed amount of GroEL (1 μM) were added to immobilized host proteins. Concentrations are indicated below the graphs. Bound GroEL and LigBC were detected using mouse anti-GroEL (black boxes) and anti-LigBC (gray boxes), respectively, followed by peroxidase-conjugated secondary antibodies. Optical densities were determined at 492 nm. Each point represents the mean absorbance value at 492 nm ± standard deviation of three independent experiments