Fig. 6

Binding of GroEL to host molecules as a function of protein concentration. The dose-dependent binding was determined by varying the protein concentration from 0 to 2 μM. The absorbance values obtained with BSA were subtracted from values obtained with the binding of recombinant proteins to host components. Each point represents the mean absorbance value at 492 nm ± standard deviation of three independent experiments. Lp25 and LigBC were used as negative and positive binding controls, respectively