Fig. 10

Cytokine secretion assay. J774A.1 cells were stimulated (20 h of incubation at 37 °C) with GroEL (10 μg/mL) and LPS (50 ng/mL) treated with 50 μg/mL of polymyxin B or 50 μg/mL of proteinase K. Data represent the mean of IL-6 (a) and TNF-α. (b) concentrations in the cellular supernatant ± standard error of three independent experiments. Values of p < 0.05 (*) were considered statistically significant, when compared to the binding of the cells did not stimulate (medium) by t-test