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Fig. 6 | BMC Microbiology

Fig. 6

From: Co-expression of double-stranded RNA and viral capsid protein in the novel engineered Escherichia coli DualX-B15(DE3) strain

Fig. 6

dsRNA-VP28 and MrNV-CP could be co-expressed in DualX-B15(DE3). a The RNase digestion assays: untreated (U), RNase A (A) and RNase III (III)-treated samples. Lane M (Marker) is a 2-log DNA ladder. The molecular weight of dsRNA-VP28 is indicated by an arrow. b SDS-PAGE analysis of protein expression in the three different strains of E. coli. A red dot is shown to the right of the expected band of MrNV-CP. c Western blot was performed with anti-MrNV capsid protein monoclonal antibody. Lane M: a pre-stained protein marker; Lane N: cells without the protein-expression plasmid; Lane I: induced cells; Lane P: a positive control (purified MrNV capsid proteins); Lane INS: insoluble fraction; Lane SOL: soluble fraction. The molecular weight of MrNV capsid protein is 40 kDa as indicated by a red asterisk.

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