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Fig. 1 | BMC Microbiology

Fig. 1

From: Co-expression of double-stranded RNA and viral capsid protein in the novel engineered Escherichia coli DualX-B15(DE3) strain

Fig. 1

Construction of the RNase III- and protease-deficient DualX-B15(DE3) strain by P1 phage transduction. a Infection of the donor strain HT115(DE3) by P1 bacteriophage. b Random incorporation of the donor genome into viral capsids. c Release of new viral particles upon lysis of the donor cells. d Infection of the recipient strain BL21(DE3) with P1 lysate. e Homologous recombination at the rnc14 gene locus. f Generation of the novel E. coli strain DualX-B15(DE3) with a tetracycline resistant marker (TetR)

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BMC Microbiology

ISSN: 1471-2180

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