Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP

Table 1 Bacteria used in the current study

Strains	Relevant characteristics	Source
Pseudomonas aeruginosa
PAO1	wild type	[32]
∆aauR PAO1	∆aauR derivative of PAO1	This study
∆aatQM PAO1	∆aatQM derivative of PAO1	This study
∆aatJ PAO1	∆aatJ derivative of PAO1	This study
rpoN:Ω-Km PAO1	ΔrpoN derivative of PAO1	[32]
PA14	wild type	[39]
∆aauR PA14	∆aauR derivative of PA14	This study
PAK	wild type	[40]
∆aauR PAK	∆aauR derivative of PAK	This study
Escherichia coli MG1655	F⁻ λ⁻ ilvG⁻ rfb-50 rph-1	[41]
Escherichia coli Top10^a	F⁻ mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ (ara-leu)7697 galE15 galK16 rpsL(Str^r) endA1 λ⁻	Invitrogen

ISSN: 1471-2180