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Table 1 Bacteria used in the current study

From: Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP

Strains Relevant characteristics Source
Pseudomonas aeruginosa
 PAO1 wild type [32]
 ∆aauR PAO1 aauR derivative of PAO1 This study
 ∆aatQM PAO1 aatQM derivative of PAO1 This study
 ∆aatJ PAO1 aatJ derivative of PAO1 This study
rpoN:Ω-Km PAO1 ΔrpoN derivative of PAO1 [32]
 PA14 wild type [39]
 ∆aauR PA14 aauR derivative of PA14 This study
 PAK wild type [40]
 ∆aauR PAK aauR derivative of PAK This study
Escherichia coli MG1655 F λ ilvG rfb-50 rph-1 [41]
Escherichia coli Top10a F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ (ara-leu)7697 galE15 galK16 rpsL(Strr) endA1 λ Invitrogen
  1. aUsed for cloning purposes and plasmid maintenance