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Table 1 Bacteria used in the current study

From: Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP

Strains

Relevant characteristics

Source

Pseudomonas aeruginosa

 PAO1

wild type

[32]

 ∆aauR PAO1

aauR derivative of PAO1

This study

 ∆aatQM PAO1

aatQM derivative of PAO1

This study

 ∆aatJ PAO1

aatJ derivative of PAO1

This study

rpoN:Ω-Km PAO1

ΔrpoN derivative of PAO1

[32]

 PA14

wild type

[39]

 ∆aauR PA14

aauR derivative of PA14

This study

 PAK

wild type

[40]

 ∆aauR PAK

aauR derivative of PAK

This study

Escherichia coli MG1655

F λ ilvG rfb-50 rph-1

[41]

Escherichia coli Top10a

F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ (ara-leu)7697 galE15 galK16 rpsL(Strr) endA1 λ

Invitrogen

  1. aUsed for cloning purposes and plasmid maintenance