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Fig. 6 | BMC Microbiology

Fig. 6

From: Utilization of L-glutamate as a preferred or sole nutrient in Pseudomonas aeruginosa PAO1 depends on genes encoding for the enhancer-binding protein AauR, the sigma factor RpoN and the transporter complex AatJQMP

Fig. 6

Genetic-complementation experiments for the ΔaauR mutant grown on L-glutamate (a) and L-glutamine (b) as carbon sources. The ΔaauR mutant was transformed with expression plasmids encoding for aauR (aauR-pBBR1MCS-5), aatJ (aatJ-pBBR1MCS-5), aatQMP (aatQMP-pBBR1MCS-5) or aatJQMP (aatJQMP-pBBR1MCS-5). Both wild-type P. aeruginosa PAO1 and the ΔaauR mutant harboring empty plasmid (pBBR1MCS-5) were included as controls. Bacteria were grown at 37 °C in minimal media supplemented with 30 μg mL− 1 gentamicin, 10 mM NH4Cl and 20 mM of indicated amino acid as the sole carbon source. Data points represent mean values (n = 3) ± SD

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BMC Microbiology

ISSN: 1471-2180

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