Identification of cutaneous fungi and mites in adult atopic dermatitis: analysis by targeted 18S rRNA amplicon sequencing

Table 2 Presence of Demodex folliculorum and Geotrichum candidum on skin

Species		Observed^a (no. [%])		OR (95% CI)^b	Adjusted p-value^c
Species		Presence	Absence	OR (95% CI)^b	Adjusted p-value^c
Demodex folliculorum	Control skin	7 (15%)	39 (85%)	1
	AD LS	33 (60%)	22 (40%)	8.2 (2.9–25.7)	< 0.003
	AD NLS	38 (69%)	17 (31%)	12.1 (4.3–38.8)	< 0.001
Geotrichum candidum	control skin	2 (4%)	44 (96%)	1
	AD LS	20 (36%)	35 (64%)	12.3 (2.7–115.8)	0.04
	AD NLS	19 (35%)	36 (65%)	11.4 (2.5–107.2)	0.07

a: Species presence was defined as 10 ≥ classified reads in a sample. b: Pairwise differences between sample sites were tested using Fisher’s exact test and odds ratios were calculated using healthy control skin from non-AD individuals as the reference. c: P-values were corrected for mass-significance using the Bonferroni method. Adjusted p-values < 0.05 were considered as statistically significant. Abbreviations: AD atopic dermatitis, LS lesional skin, NLS non-lesional skin, OR odds ratio, CI confidence interval

ISSN: 1471-2180