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Table 1 Strains and plasmids used in the study

From: FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

Strain or plasmid Relevant properties Source or reference
A. caulinodans ORS571 Wild-type strain, Ampr, Nalr [1]
 ΔacfR ORS571 derivative, acfR deletion mutant, Ampr, Nalr This study
 ΔacfR-C ΔacfR mutant harboring the pBBR-acfR plasmid, Ampr, Nalr, Kanr This study
E. coli DH5a FS--upE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Transgen
 pCM351 Mobilizable allelic exchange vector, Ampr, Genr [23]
 pCM157 IncP plasmid that expresses Cre recombinase, Tetr [23]
 pRK2013 Helper plasmid, ColE1 replicon, Tra+, Kanr [24]
 pBBR1MCS-2 Broad-host-range cloning vector, Kanr [25]
 pBBR-acfR pBBR1MCS-2 with acfR ORF and upstream promoter region, Kanr This study
  1. Ampr ampicillin resistance, Nalr Nalidixic acid, Genr gentamicin resistance, Kanr kanamycin resistance, Tetr tetracycline resistance