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Table 1 Strains and plasmids used in the study

From: FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant

Strain or plasmid

Relevant properties

Source or reference

Strain:

A. caulinodans ORS571

Wild-type strain, Ampr, Nalr

[1]

 ΔacfR

ORS571 derivative, acfR deletion mutant, Ampr, Nalr

This study

 ΔacfR-C

ΔacfR mutant harboring the pBBR-acfR plasmid, Ampr, Nalr, Kanr

This study

E. coli DH5a

FS--upE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Transgen

Plasmid:

 pCM351

Mobilizable allelic exchange vector, Ampr, Genr

[23]

 pCM157

IncP plasmid that expresses Cre recombinase, Tetr

[23]

 pRK2013

Helper plasmid, ColE1 replicon, Tra+, Kanr

[24]

 pBBR1MCS-2

Broad-host-range cloning vector, Kanr

[25]

 pBBR-acfR

pBBR1MCS-2 with acfR ORF and upstream promoter region, Kanr

This study

  1. Ampr ampicillin resistance, Nalr Nalidixic acid, Genr gentamicin resistance, Kanr kanamycin resistance, Tetr tetracycline resistance