From: FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant
Strain or plasmid | Relevant properties | Source or reference |
---|---|---|
Strain: | ||
A. caulinodans ORS571 | Wild-type strain, Ampr, Nalr | [1] |
ΔacfR | ORS571 derivative, acfR deletion mutant, Ampr, Nalr | This study |
ΔacfR-C | ΔacfR mutant harboring the pBBR-acfR plasmid, Ampr, Nalr, Kanr | This study |
E. coli DH5a | F−S--upE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Transgen |
Plasmid: | ||
pCM351 | Mobilizable allelic exchange vector, Ampr, Genr | [23] |
pCM157 | IncP plasmid that expresses Cre recombinase, Tetr | [23] |
pRK2013 | Helper plasmid, ColE1 replicon, Tra+, Kanr | [24] |
pBBR1MCS-2 | Broad-host-range cloning vector, Kanr | [25] |
pBBR-acfR | pBBR1MCS-2 with acfR ORF and upstream promoter region, Kanr | This study |