TY - JOUR AU - Baishya, Somorita AU - Deshamukhya, Chandrayee AU - Wangkheimayum, Jayalaxmi AU - Das, Bhaskar Jyoti AU - Anbarasu, Anand AU - Talukdar, Anupam Das AU - Bhattacharjee, Amitabha AU - Choudhury, Manabendra Dutta PY - 2021 DA - 2021/03/09 TI - Transcriptional expression of secondary resistance genes ccdB and repA2 is enhanced in presence of cephalosporin and carbapenem in Escherichia coli JO - BMC Microbiology SP - 79 VL - 21 IS - 1 AB - The issue of carbapenem resistance in E.coli is very concerning and it is speculated that cumulative effect of both primary resistance genes and secondary resistance genes that act as helper to the primary resistance genes are the reason behind their aggravation. Therefore, here we attempted to find the role of two secondary resistance genes (SRG) ccdB and repA2 in carbapenem resistance in E. coli (CRE). In this context influential genes belonging to secondary resistome that act as helper to the primary resistance genes like blaNDM and blaCTX-M in aggravating β-lactam resistance were selected from an earlier reported in silico study. Transcriptional expression of the selected genes in clinical isolates of E.coli that were discretely harboring blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7 and blaCTX-M-15 with and without carbapenem and cephalosporin stress (2 μg/ml) was determined by real time PCR. Cured mutants sets that were lacking (i) primary resistance genes, (ii) secondary resistance genes and (iii) both primary and secondary resistance genes were prepared by SDS treatment. These sets were then subjected to antibiotic susceptibility testing by Kirby Bauer disc diffusion method. SN - 1471-2180 UR - https://doi.org/10.1186/s12866-021-02136-y DO - 10.1186/s12866-021-02136-y ID - Baishya2021 ER -