Detection and characterization of carbapenem resistant Gram‐negative bacilli isolates recovered from hospitalized patients at Soba University Hospital, Sudan

Table 1 Frequency of carbapenemase producing among Gram-negative bacilli by phenotypic tests

	Positive isolates for a particular phenotypic test				Total
Bacterial isolates	EDTA	MHT + BA	EDTA + BA	EDTA + AB + MHT
	n (%)	n (%)	n (%)	n (%)	n (%)
E.coli (n = 28)	17 (16.4)	2 (8.3)	3 (11.1)	4 (26.6)	26 (15.2)
Klebsiella spp. (n = 82)	41 (39)	10 (41.6)	12 (44.4)	8 (53.3)	71 (41.5)
Pseudomonas spp. (n = 45)	19 (18)	9 (37.5)^a	4 (14.9)	1 (6.6)	33(19.2)
Acin. baumannii (n = 36)	19 (18)	3 (12.6) ^a	6 (22.2)	2 (13.3)	30 (17.6)
Burkholderia cepacia (n = 2)	2 (1.9)	0 (0)	0 (0)	0 (0)	2 (1.2)
Enterobacter spp. (2)	2 (1.9)	0 (0)	0 (0)	0 (0)	2 (1.2)
Other GNB^b (n = 11)	5 (4.8)	0 (0)	2 (7.4)	0(0)	7 (4)
Total (n = 206)	105(50.9)	24 (11.7)	27 (13.1)	15 (7.2)	171(83)

-MHT positive = KPC + OXA48 - Boronic acid positive = KPC
-MHT + Boronic acid positive = KPC -MHT positive + boronic acid negative = OXA 48
Note: MHT applied only for Enterobacterceae. -EDTA positive = Metallo-β-lactamase^.aThese isolates just positive by BA and not MHT.^bOther Gram-negative bacilli include: Citrobacter species (3), Serratia species (1), Proteus spp. (2), Stenotrophomonas maltophilia (3), Vibrio vurneficus (1) and Morganella morganii (1).

ISSN: 1471-2180