Fig. 2

Results of genome re-sequencing of a typical false positive clone isolated during attempts to delete a ~ 68 kb region (M08). The red box indicates an unintended deletion of ~ 47 kb located between MSR1_03260 and MSR1_03780 (nt position 360,736). Brackets indicate parts of the remaining suicide vector with the Kmr marker and the galK gene inactivated by a spontaneous insertion of a tandem IS element (green arrows), as well as an unintended insertion of several mam genes (mamH, mamI and parts of mamE) next to the remnants of the suicide vector