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Fig. 2 | BMC Microbiology

Fig. 2

From: Two novel XRE-like transcriptional regulators control phenotypic heterogeneity in Photorhabdus luminescens cell populations

Fig. 2

Phenotypic differences in P. luminescens 1° and 2° cells as well as xreR1 and xreR2 mutant and overproduction strains. a Pigmentation, bioluminescence, antibiotic (AB) production and mucoid colony forming of P. luminescens 1° and 2° wildtype, P. luminescens 1° ΔxreR1 and P. luminescens 2° ΔxreR2 mutant strains, as well as P. luminescens strains that carry chromosomally integrated extra copies of xreR1 in 2° cells (2° + PconstxreR1) and of xreR2 in 1° cells (1° + PconstxreR2), respectively, each under the control of the constitutive promoter Ptac overproducing the XRE-regulator of the respective other cell variant. b Nematode symbiosis bioassays. Number of H. bacteriophora hermaphrodites developed on P. luminescens 1° and 2° wildtype (wt) as well as P. luminescens 1° ΔxreR1 and P. luminescens 2° ΔxreR2 mutant strains after 7 days (left panel), and the number of the respective P. luminescens cells isolated from the infective juveniles (IJ) given in colony forming units (CFUs) per IJ after 21 days (right panel). The asterisks (***) indicate statistically significant differences with a p-value smaller than 0.001. ns = not significant. Error bars represent standard error of three independently performed experiments

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