Fig. 1
From: Activation of short-chain ketones and isopropanol in sulfate-reducing bacteria
Representative HPLC-MS chromatograms of an in vitro enzyme assay of the B12-dependent 2-hydroxyisobutyryl-CoA mutase reaction. Time course measurement of the reaction showing formation of 3-hydroxybutyryl-CoA (peak eluting between 5.87 and 6.21 min), concomitant with disappearance of 2-hydroxyisobutyryl-CoA (peak eluting between 6.24 and 6.62 min). The product 3-hydroxybutyryl-CoA eluted before the substrate 2-hydroxyisobutyryl-CoA