Fig. 5

TMMI-012 induces severe scedosporiosis in mice than S. boydii. a After inoculation of S. boydii for a week, intact mice brain and lung are shown (scale bar ~ 20 μm). b After inoculation of TMMI-012 for a week, mice brain shown cerebral lesion with neuronal degradation (upper left and lower left) and hemorrhage (upper right) (scale bar ~ 20 μm). Asterisk (*) indicates neuronal degradation area; Number sign (#) indicates hemorrhage area. Mice alveolar shown alveolar septal thickening (upper). Arrow indicates accumulation of fungal hyphae. Positive stain of Grocott’s methenamine-silver is shown both in brain (lower right) and in lung tissue indicating the accumulation of fungal hyphae (scale bar ~ 10 μm). c Histological scores of brain and lung of mice infected with S. boydii and TMMI-012. d Representative image of immunofluorescence staining for water homeostasis marker (AQP-4) in cerebral cortex of mice infected with S. boydii and TMMI-012 (scale bar ~ 20 μm). Inserts depict high magnification of the AQP-4 immunofluorescence stain (scale bar ~ 10 μm). e Quantification of H-score of AQP-4 immunofluorescence in cerebral cortex of mice infected with S. boydii and TMMI-012. Data shown as mean ± SD (n = 3); P-value calculated by Student’s t-test; *P < 0.05