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Table 3 Statistics of MiSeq™ sequencing data for human fecal samples

From: Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

Sample

Paired reads

Merged reads

Filtered reads

Q Score

No. of reads

No. of reads

Avg (bp)

No. of reads

Avg (bp)

F1/I

V3-V4

66,242

63,821

449.3

63,778 (96.3%)

449.5

31.4

F2/I

V3-V4

68,824

66,640

447.6

66,490 (96.6%)

448.3

32.3

F3/I

V3-V4

132,057

128,095

446.9

127,999 (96.9%)

447.1

31.6

F4/I

V3-V4

103,532

100,945

451.4

100,853 (97.4%)

451.7

32.8

F5/I

V3-V4

72,136

70,521

451

70,459 (97.7%)

451.3

33.2

F6/I

V3-V4

52,182

50,907

449

50,841 (97.4%)

449.5

33.2

  1. I: Illumina MiSeq™, Avg: average read length, Q score: average Phred quality score. The percentage of reads retained after size filtering is shown in parentheses