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Table 3 Statistics of MiSeq™ sequencing data for human fecal samples

From: Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

Sample Paired reads Merged reads Filtered reads Q Score
No. of reads No. of reads Avg (bp) No. of reads Avg (bp)
F1/I
V3-V4
66,242 63,821 449.3 63,778 (96.3%) 449.5 31.4
F2/I
V3-V4
68,824 66,640 447.6 66,490 (96.6%) 448.3 32.3
F3/I
V3-V4
132,057 128,095 446.9 127,999 (96.9%) 447.1 31.6
F4/I
V3-V4
103,532 100,945 451.4 100,853 (97.4%) 451.7 32.8
F5/I
V3-V4
72,136 70,521 451 70,459 (97.7%) 451.3 33.2
F6/I
V3-V4
52,182 50,907 449 50,841 (97.4%) 449.5 33.2
  1. I: Illumina MiSeq™, Avg: average read length, Q score: average Phred quality score. The percentage of reads retained after size filtering is shown in parentheses