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Table 2 Statistics of MinION™ sequencing data for human fecal samples

From: Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

Sample Pass reads Trimmed reads Filtered reads
No. of reads Min (bp) Avg (bp) Max (bp) No. of reads Avg (bp) No. of reads Avg (bp) Q score
F1/N
V1-V9
104,895 186 1521.1 4549 103,100 1386.4 89,752 (85.6%) 1463.7 9.4
F2/N
V1-V9
84,065 169 1393.8 4253 82,458 1259.8 60,326 (71.8%) 1461.4 9.4
F3/N
V1-V9
76,968 168 1474.3 4829 74,479 1343.3 60,713 (78.9%) 1465.5 9.4
F4/N
V1-V9
114,060 168 1541.7 4836 111,436 1410.6 100,569 (88.2%) 1469.9 9.4
F5/N
V1-V9
85,912 177 1536.0 4877 83,038 1405.4 74,168 (86.3%) 1474.2 9.4
F6/N
V1-V9
108,938 213 1525.1 4866 106,857 1393.5 93,146 (85.5%) 1467.4 9.4
F1/N
V3-V4
52,864 160 568.8 2759 52,283 435.2 48,494 (91.7%) 442.5 9.1
F2/N
V3-V4
92,816 174 583.4 2886 91,989 442.8 89,016 (95.9%) 444.7 9.2
F3/N
V3-V4
60,200 163 568.5 2062 59,435 434.6 55,706 (92.5%) 441.1 9.2
F4/N
V3-V4
83,021 202 578.0 2050 81,734 446.1 77,995 (93.9%) 450.0 9.2
F5/N
V3-V4
78,409 167 578.4 1796 76,135 447.8 72,526 (92.5%) 453.1 9.2
F6/N
V3-V4
74,931 114 580.3 2246 73,946 446.1 71,330 (95.2%) 449.1 9.2
  1. N: Oxford Nanopore MinION™, Min: minimum read length, Avg: average read length, Max: maximum read length, Q score: average Phred quality score. The percentage of reads retained after size filtering is shown in parentheses