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Table 2 Statistics of MinION™ sequencing data for human fecal samples

From: Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

Sample

Pass reads

Trimmed reads

Filtered reads

No. of reads

Min (bp)

Avg (bp)

Max (bp)

No. of reads

Avg (bp)

No. of reads

Avg (bp)

Q score

F1/N

V1-V9

104,895

186

1521.1

4549

103,100

1386.4

89,752 (85.6%)

1463.7

9.4

F2/N

V1-V9

84,065

169

1393.8

4253

82,458

1259.8

60,326 (71.8%)

1461.4

9.4

F3/N

V1-V9

76,968

168

1474.3

4829

74,479

1343.3

60,713 (78.9%)

1465.5

9.4

F4/N

V1-V9

114,060

168

1541.7

4836

111,436

1410.6

100,569 (88.2%)

1469.9

9.4

F5/N

V1-V9

85,912

177

1536.0

4877

83,038

1405.4

74,168 (86.3%)

1474.2

9.4

F6/N

V1-V9

108,938

213

1525.1

4866

106,857

1393.5

93,146 (85.5%)

1467.4

9.4

F1/N

V3-V4

52,864

160

568.8

2759

52,283

435.2

48,494 (91.7%)

442.5

9.1

F2/N

V3-V4

92,816

174

583.4

2886

91,989

442.8

89,016 (95.9%)

444.7

9.2

F3/N

V3-V4

60,200

163

568.5

2062

59,435

434.6

55,706 (92.5%)

441.1

9.2

F4/N

V3-V4

83,021

202

578.0

2050

81,734

446.1

77,995 (93.9%)

450.0

9.2

F5/N

V3-V4

78,409

167

578.4

1796

76,135

447.8

72,526 (92.5%)

453.1

9.2

F6/N

V3-V4

74,931

114

580.3

2246

73,946

446.1

71,330 (95.2%)

449.1

9.2

  1. N: Oxford Nanopore MinION™, Min: minimum read length, Avg: average read length, Max: maximum read length, Q score: average Phred quality score. The percentage of reads retained after size filtering is shown in parentheses