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Fig. 4 | BMC Microbiology

Fig. 4

From: Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

Fig. 4

Comparison of taxonomic profiles of human gut microbiota between sequencing methodologies. Six fecal samples (F1-F6) were analyzed by sequencing the entire 16S rRNA gene using MinION™ (N_V1-V9). For comparison, the V3-V4 region was sequenced on MinION™ (N_V3-V4) or MiSeq™ platforms (I_V1-V9). Randomly sampled 20,000 reads from each data set were allocated to the reference genome database of 5850 representative bacterial species. A heat map shows the relative genus abundance (%) of classified reads. The 15 most abundant taxa are shown. The Pearson correlation coefficient (r) between sequencing methods was computed. Asterisks indicate significant correlations at P < 0.05

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BMC Microbiology

ISSN: 1471-2180

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