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Table 1 D-optimal design showing the experimental runs carried out and the observed and predicted responses

From: Production and statistical optimization of Paromomycin by Streptomyces rimosus NRRL 2455 in solid state fermentation

Run Order

pH (A)

Temperature (B, °C)

Inoculum size (C, % v/w)

IZ diameter (mm)

Observed response

Predicted response

1

8.50

37.00

5.00

20

19.79

2

8.50

23.00

5.00

21

20.82

3

5.50

37.00

5.00

0

0.04

4

8.50

37.00

35.00

21

20

5

8.50

23.00

35.00

18

18.35

6

6.25

30.00

12.50

23

21.26

7

5.50

37.00

35.00

0

0.2

8

8.50

37.00

5.00

19

19.79

9

7.00

30.00

20.00

24

23.83

10

5.50

23.00

20.00

21

20.52

11

7.00

30.00

35.00

22

23.27

12

5.50

23.00

5.00

21

21.75

13

8.50

23.00

35.00

19

18.35

14

5.50

37.00

35.00

0

0.21

15

7.00

23.00

5.00

23

23.2

16

8.50

30.00

20.00

26

26.64