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Fig. 7 | BMC Microbiology

Fig. 7

From: Zinc limitation in Klebsiella pneumoniae profiled by quantitative proteomics influences transcriptional regulation and cation transporter-associated capsule production

Fig. 7

In vitro macrophage cytotoxicity assay of K. pneumoniae. a Cytotoxicity assay of Balb/C macrophages co-cultured with K. pneumoniae WT and ∆chaB following initial bacterial culturing in TSB media (nutrient rich). b Cytotoxicity assay of Balb/C macrophages co-cultured with K. pneumoniae WT and ∆chaB following initial bacterial culturing in M9 media (nutrient poor). Two independent deletion mutants of chaB were characterized across all experiments. Results reflect the average of three biological replicates and technical duplicates. Error bars depict standard deviation. *denotes significant difference between WT and mutant strains by Student’s t-test, p-value ≤ 0.05

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